Table 1

ISSR primers designed for this study

Primer

Sequence

Repeat Type


ISSR_7

DDB(agg)5

Trinucleotide

ISSR_8

DDB(cag)5

Trinucleotide

ISSR_9

DDB(gag)5

Trinucleotide

ISSR_10

DDB(ctc)5

Trinucleotide

ISSR_11

DDB(gtg)5

Trinucleotide

ISSR_12

DDB(aacg)4

Tetranucleotide

ISSR_13

DDB(cgca)4

Tetranucleotide

ISSR_14

DDB(gcca)4

Tetranucleotide

ISSR_15

DDB(ct)8

Dinucleotide

ISSR_16

DDB(ca)8

Dinucleotide


Capital letters in ISSR primer sequences denote degenerate sites: B denotes nucleotides c, g, or t; D denotes a, g, or t. Subscripts indicate the number of repeats. Bold lettering indicates the primers used for fingerprinting.

Neal et al. BMC Microbiology 2011 11:203   doi:10.1186/1471-2180-11-203

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