Table 3

Putative EPS genes in H.somni 2336 and 129Pt with proposed roles in polysaccharide synthesis

Gene

ORF

(HSM-H. somni 2336 and HS- H. somni 129Pt)

Protein annotation

No. of amino acids, predicted mass (kDa)

% Similarity to another protein


galU

HSM_1063

HS_1117

UTP-glucose-1-phosphate uridylyltransferase

295, 32.2

70, to glucose-1-phosphate uridylyltransferase, galU (E. coli)

manB

HSM_1062

HS_1118

Phosphomannomutase

454, 50.3

81, to phosphomannomutase, cpsG (E. coli)

csrA

HSM_1061

HS_1119

Carbon storage regulator

60, 6.75

89, to pleiotropic regulatory protein for carbon source metabolism, csrA (E. coli)

pldB

HSM_1242

HS_0775

Lysophospholipase

318, 37.4

49, to lysophospholipase L2, pldB (E. coli)

ybhA

HSM_1241

HS_0774

Haloacid dehalogenase-like hydrolase

273, 30.8

60, to phosphatase//phospho transferase, ybhA (E. coli)

araD

HSM_1240

HS_0773

L-ribulose-5-phosphate 4-epimerase

231, 25.8

82, to L-ribulose-5-phosphate 4-epimerase, yiaS (E. coli)

sgbU

HSM_1239

HS_0772

Putative L-xylulose-5-phosphate 3-epimerase

290, 33.2

84, to L-xylulose 5-phosphate 3-epimerase, yiaQ (E. coli)

rmpA

HSM_1238

HS_0771

3-keto-L-gulonate-6-phosphate decarboxylase

215, 23.6

64, to 3-keto-L-gulonate 6-phosphate decarboxylase, yiaQ (E. coli)

xylB

HSM_1237

HS_0770

L-xylulose kinase

484, 53.7

75, to L-xylulose kinase, lyxK (E. coli)

rbs1C

HSM_1236

HS_0769

Ribose ABC transporter, permease

342, 32.9

59, to D-ribose transporter subunit, rbsc (E. coli)

rbs1A

HSM_1235

HS_0768

Ribose ABC transporter, ATPase component

496, 56.1

60, to D-ribose transporter subunit, ATP-binding component, rbsA (E. coli K12)

rbs1B

HSM_1234

HS_0767

ABC-type sugar transport system, periplasmic component

312, 31.0

56, to D-ribose transporter subunit, periplasmic component (E. coli )

glsS

HSM_1233

HS_0766

Gluconolaconase

295, 32.6

46, to gluconolactonase, gnl

(Zymomonas mobilis)

rbs2B

HSM_1232

HS_0765

ABC-type sugar-binding periplasmic protein

369, 37.2

81, to hypothetical protein (Yersinia intermedia ATCC 29909)

rbs2C

HSM_1231

HS_0764

Ribose ABC transporter, permease

349, 36.9

90, to inner-membrane translocator (Yersinia intermedia ATCC 29909)

rbs2A

HSM_1230

HS_0763

Ribose ABC transporter, ATPase component

505, 55.8

81, to ABC transporter-related protein (Yersinia intermedia ATCC 29909)

dctP

HSM_1229

HS_0762

TRAP C4-dicarboxylate transport system, periplasmic component

328, 33.4

52, to C4-dicarboxylate binding protein, periplasmic component, dctP (Rhodobacter capsulatus)

dctM

HSM_1228

HS_0761

TRAP C4-dicarboxylate transport system, permease component

426, 43.2

59, to C4-dicarboxylate -binding protein, permease component, dctM (Rhodobacter capsulatus)

dctQ

HSM_1227

HS_0760

Tripartite ATP-independent periplasmic transporter

160, 17.8

40, to tripartite ATP-independent periplasmic transporter, dctQ

(Rhodobacter capsulatus)


Sandal et al. BMC Microbiology 2011 11:186   doi:10.1186/1471-2180-11-186

Open Data