Open Access Highly Accessed Research article

Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation

Indra Sandal15, Thomas J Inzana1*, Antonio Molinaro2, Christina De Castro2, Jian Q Shao3, Michael A Apicella3, Andrew D Cox4, Frank St Michael4 and Gretchen Berg16

  • * Corresponding author: Thomas J Inzana tinzana@vt.edu

  • † Equal contributors

Author Affiliations

1 Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, USA

2 Department of Organic Chemistry and Biochemistry University of Naples "Federico II", Italy

3 Department of Microbiology, University of Iowa, Iowa City, IA, USA

4 National Research Council, Ontario, Canada

5 Division of Pulmonary Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA

6 Department of Medicine, Boston University School of Medicine, Boston, MA

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BMC Microbiology 2011, 11:186  doi:10.1186/1471-2180-11-186

Published: 19 August 2011

Additional files

Additional file 1:

Proposed composition of OdA LOS from H. somni strains 2336 and 129Pt grown as a biofilm, as planktonic cells, or on blood agar plates by negative-ion-ES-MS. Data of the observed ions (m/z), observed and calculated molecular mass (in daltons), and proposed composition of O-deacylated lipooligosaccharides from H. somni strains 2336, which can be sialylated, and 129Pt, which is cannot be sialylated, grown with and without sialic acid as a biofilm, planktonically, and on blood agar.

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Additional file 2:

Maps of H. somni 2336 chromosomal loci containing genes proposed to encode for proteins involved in EPS biosynthesis. A, an ~19 kb region containing genes predicted to encode for glycosyltransferases and transport proteins; B, an ~3 kb region that contains manB. For detailed analyses of the putative gene products see Table 3.

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Open Data