Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation
- Equal contributors
1 Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, USA
2 Department of Organic Chemistry and Biochemistry University of Naples "Federico II", Italy
3 Department of Microbiology, University of Iowa, Iowa City, IA, USA
4 National Research Council, Ontario, Canada
5 Division of Pulmonary Medicine, University of Utah School of Medicine, Salt Lake City, UT, USA
6 Department of Medicine, Boston University School of Medicine, Boston, MA
BMC Microbiology 2011, 11:186 doi:10.1186/1471-2180-11-186Published: 19 August 2011
Additional file 1:
Proposed composition of OdA LOS from H. somni strains 2336 and 129Pt grown as a biofilm, as planktonic cells, or on blood agar plates by negative-ion-ES-MS. Data of the observed ions (m/z), observed and calculated molecular mass (in daltons), and proposed composition of O-deacylated lipooligosaccharides from H. somni strains 2336, which can be sialylated, and 129Pt, which is cannot be sialylated, grown with and without sialic acid as a biofilm, planktonically, and on blood agar.
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Additional file 2:
Maps of H. somni 2336 chromosomal loci containing genes proposed to encode for proteins involved in EPS biosynthesis. A, an ~19 kb region containing genes predicted to encode for glycosyltransferases and transport proteins; B, an ~3 kb region that contains manB. For detailed analyses of the putative gene products see Table 3.
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