ATPase activity and adhesion of M. hominis membrane proteins P50, P60/P80 and OppA variants. ATPase activities of purified proteins (0.5 μg/well) were measured in the ammonium molybdate assay as a function of ATP concentration [A.1-C.1] Protein adhesion to HeLa cells was measured in cell-ELISA [A.2-C.2]. A comparison of the relative ATPase activity (black bars) and adhesion (striped bars) with regard to wild type OppA is shown in [A.3-C.3]. Data represent means of three independent experiments with triplicate samples in each experiment. Statistical analysis was performed by unpaired t-test and statistically significant results designated by *. *P < 0.05, **P < 0.01, and ***P < 0.001. The ATPase activity or adhesion of the OppA mutants were compared with those of the recombinant OppA (R).
Hopfe et al. BMC Microbiology 2011 11:185 doi:10.1186/1471-2180-11-185