OM proteome analysis following cold shock in M. catarrhalis. OMPs were extracted from a culture of M. catarrhalis strain O35E, which was exposed to a 3-hour cold shock at 26°C (A) or to continuous growth at 37°C (B). A collection of 6 gels (3 of each temperature) resulting from three independent experiments was analyzed by ImageMaster® 2D Platinum software (Amersham). Three OMPs that are differentially regulated in response to a 26°C cold shock, are indicated in the boxes (A and B). Gel of OMPs isolated from a M. catarrhalis O35E.tbpB mutant grown at 37°C is shown (C). Identified proteins are labeled. The pI and mass (kDa) values are shown at the top and the right side of each gel.
Spaniol et al. BMC Microbiology 2011 11:182 doi:10.1186/1471-2180-11-182