Macrophage death upon contact with the C. parapsilosis environmental strain CarcC. To detect dead phagocytes, cells were incubated with 1 μg/ml of PI and visualized at 1, 2, 4, 8, and 12 hours of infection with a magnification of 200 ×. Images were obtained using fluorescent microscopy (c), bright-field microscopy (b) and the superposition of both (a). At each time point, dead macrophages without C. parapsilosis served as negative control (d). C. parapsilosis cell morphology in the absence of macrophages (e).
Sabino et al. BMC Microbiology 2011 11:180 doi:10.1186/1471-2180-11-180