Macrophage death upon contact with the C. parapsilosis blood isolate 972697. To detect dead phagocytes, cells were incubated with 1 μg/ml of PI and visualized at 1, 2, 4, 8, and 12 hours of infection with a magnification of 200 ×. Necrotic nuclei are presented in red. Images were obtained using fluorescent microscopy (c), bright-field microscopy (b) and the superposition of both (a). At each time point, dead macrophages without C. parapsilosis served as negative control (d). C. parapsilosis cell morphology in the absence of macrophages (e).
Sabino et al. BMC Microbiology 2011 11:180 doi:10.1186/1471-2180-11-180