Figure 1.

Growth kinetics of the galU mutant in vitro. Growth of wild-type, galU mutant, and galU-complemented strains of FT after 48 hrs of culture was measured by the gradient plating technique to determine their sensitivity to polymyxin-B and deoxycholate. All data points represent the mean (± SEM) of triplicate samples. Statistical analyses were performed via one-way ANOVA with Bonferroni post-tests. Statistically significant differences are indicated as follows: P < 0.01 (**) (Panel A). Growth of each strain cultured in MHB supplemented with either 0.1% glucose or 2% D-galactose (Panel B) or within macrophage-like murine cell lines (J774 or RAW264.7 at an MOI of 10, Panel C) was monitored over a 24 hour period. All data points represent the mean (± SEM) of triplicate samples. Each panel is representative of at least three experiments of similar design. Statistical analyses were performed via two-way ANOVA with Bonferroni post-tests. Statistically significant differences are indicated as follows: P < 0.01 (**) and P < 0.001 (***).

Jayakar et al. BMC Microbiology 2011 11:179   doi:10.1186/1471-2180-11-179
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