Table 2

Effect of L. casei CRL 431 administration on the cytokines released in cultures of immune cells isolated from Peyer's patches of mice untreated, treated and infected with S. Typhimurium

Experimental groups

Cytokine concentration (pg/ml)


TNFα

IFNγ

IL-10


C

203 ± 32a

139 ± 83a

65 ± 13ac

Lc

257 ± 55ac

1175 ± 563bc

187 ± 91b

S

336 ± 90bcd

1384 ± 74c

102 ± 42ab

Lc-S

328 ± 4b

148 ± 86a

102 ± 24ab

Lc-S-Lc

432 ± 20d

592 ± 40b

34 ± 18c


The concentration of different cytokines were evaluated in supernatant of cultures of cells isolated from Peyer's patches of mice at 2 time points: the day of the infection (basal data) for the untreated control (C) and for mice given L. casei CRL 431 during 7 days (Lc), and 7 days post infection for infection control (S), mice given probiotic 7 days before the infection (Lc-S) and mice given continuously probiotic, before and after infection (Lc-S-Lc). Cytokine concentration in the cell culture supernatants after 24 h of incubation was determined by ELISA. Results are expressed as the means ± SD of the concentrations of each cytokine released into the supernatant (pg/ml). Means for each cytokine without a common letter differ significantly (P < 0.01).

Castillo et al. BMC Microbiology 2011 11:177   doi:10.1186/1471-2180-11-177

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