Table 1

Cytokine producing cells isolated from Peyer's patches of mice untreated or treated with L. casei CRL 431 previous and post challenge with S. Typhimurium

Experimental groups

N° of cytokine secreting cells


TNFα

IFNγ

IL-10


C

10 ± 4a

13 ± 3ad

12 ± 3a

Lc

24 ± 9b

20 ± 3b

17 ± 1b

S

26 ± 4b

16 ± 1ab

22 ± 6b

Lc-S

36 ± 12b

12 ± 2d

9 ± 4a

Lc-S-Lc

75 ± 4c

41 ± 10c

13 ± 5a,b


Cytokine producing cells were analyzed by immunocytochemistry in mononuclear cells isolated from Peyer's patches of mice at 2 time points: the day of the infection (basal data) for the untreated control (C) and for mice given L. casei CRL 431 during 7 days (Lc), and 7 days post infection for infection control (S), mice given probiotic 7 days before the infection (Lc-S), and mice given continuously probiotic, before and after infection (Lc-S-Lc). Results for healthy mice obtained the same day of the infected animals were not added because there were not significant differences compared to the basal data. Results are expressed as the means ± SD of the total number of positive cells per 2 × 104 counted cells at 1 000X magnification. Means for each value without a common letter differ significantly (P < 0.01).

Castillo et al. BMC Microbiology 2011 11:177   doi:10.1186/1471-2180-11-177

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