Table 3

Bacterial strains used in this study.

Strain

Relevant Genotypea

Source


IN56

MC4100 (λ cI857 S)

[46]

IN57

MC4100 (λ cI857 SC51S)

unpublished strain

IN61

MC4100 (λ cI857 S105C51S)

[46]

IN62

MC4100 (λ cI857 S105)

[46]

IN63

MC4100 (λ cI857 S105C51S/S76C)

[46]

IN64

MC4100 (λ cI857 SC51S/F94C)

[46]

IN65

MC4100 (λ cI857 S105C51S/F94C)

unpublished strain

IN66

MC4100 (λ cI857 SS68C)

[46]

IN67

MC4100 (λ cI857 S105C51S/I13C)

[46]

IN68

MC4100 (λ cI857 S105C51S/L14C)

[46]

IN69

MC4100 (λ cI857 SC51S/L14C)

[46]

IN70

MC4100 (λ cI857 SC51S/F78C)

unpublished strain

IN71

MC4100 (λ cI857 S105C51S/F78C)

unpublished strain

IN160

MC4100 (λ cI857 SA52G Cam)

unpublished strain

SYP026

MC4100 (λ cI857 pR'-M2), with pR' mutations

[50]

SYP027

MC4100 (λ cI857 pR'-M1), with pR' mutations

[50]

SYP028

MC4100 (λ cI857 pR'-M5), with pR' mutations

[50]

SYP043

MC4100 (λ cI857 pR'-M4), with pR' mutations

[50]


a S denotes wild-type holin gene, when expressed would produce both the S105 holin and S107 antiholin proteins. S105 signifies the mutant holin gene with its first codon altered from ATG (Met) to TTG (Leu), thus only produces the S105 holin protein.

Dennehy and Wang BMC Microbiology 2011 11:174   doi:10.1186/1471-2180-11-174

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