Additional file 2.
Figure S2. Phylogenetic analysis and sequence alignment of ferredoxins. The M. mazei and M. acetivorans sequences, labeled with the prefix MA, were derived from the CMR database . The M. thermophila (M.t.) sequence is published . The sequence of the 2 × [4Fe-4S] Clostridium pasteurianum is published  and the sequence of the 2Fe-2S Spinacia oleracea ferredoxin was obtained from the NCBI database (accession number O04683). Panel A, Phylogenetic analysis of ferredoxins. The tree was constructed by the neighbor-joining method with the MEGA4 program . Bootstrap values are shown at the nodes. Bar, evolutionary distance of 0.2. Panel B, Sequence alignment of ferredoxins from Methanosarcina species. Motifs predicted to ligate two 4Fe-4S clusters are highlighted. The alignment was performed with ClustalX2 .
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Wang et al. BMC Microbiology 2011 11:165 doi:10.1186/1471-2180-11-165