Table 1

Bacterial strains and plasmids

Strains and plasmids

Relevant genotype

Reference or source


L. monocytogenes

EGD-e

ΔtrpS × pFlo-trpS

wild-type

T. Chakraborty (University of Giessen, Germany [36]

ΔtrpS,inlA/B × pFlo-trpS

[32]

Lm-spa-

ΔtrpS,aroA,inlA/B × pFlo-trpS

This work

Lm-spa+

ΔtrpS,aroA,inlA/B,int::Phly-spa × pFlo-trpS

This work

ΔtrpS × pSP0-PactA-gfp

[36]

Lm-spa- × pSP0-PactA-gfp

ΔtrpS,aroA,inlA/B × pSP0-PactA-gfp

This work

Lm-spa+,aroA+ × pSP0-PactA-gfp

ΔtrpS,inlA/B,int::Phly-spa × pSP0-PactA-gfp

This work

Lm-spa+ × pSP0-PactA-gfp

ΔtrpS,aroA,inlA/B,int::Phly-spa × pSP0-PactA-gfp

This work


Plasmids

pFlo-trpS

TcR,

[36]

pSP0-PactA-gfp

EmR, gfp-ORF, actA-promoter

[36]

pLSV101intAB

EmR, ORIts, mutagenesis plasmid

[31]

pLSV101intAB::Phly-spa

spa-ORF, hly-promoter

This work


Heisig et al. BMC Microbiology 2011 11:163   doi:10.1186/1471-2180-11-163

Open Data