Additional file 4.

Absence of Dynabeads Protein A internalization into 4T1-HER2 cells following incubation with goat α-human Cy5 antibody. Following fixation extracellular beads were counterstained by adding Trastuzumab-Alexa488 into the supernatant. Cells were then analyzed for bead immunofluorescence using a confocal microscope. Stacked images of 5 to 16 μm tissue height were analyzed for Cy5-positive and Alexa488-negative beads. No intracellular beads were detected, indicating the lack of intrinsic bead uptake by 4T1-HER2 cells.

Format: PDF Size: 1.6MB Download file

This file can be viewed with: Adobe Acrobat Reader

Heisig et al. BMC Microbiology 2011 11:163   doi:10.1186/1471-2180-11-163