Additional file 3.
Examination of antibody binding to Dynabeads Protein A. Beads were incubated with fluorescently labeled antibodies, washed intensively to remove excess antibodies, and investigated by confocal immunofluorescence microscopy. Beads were incubated simultaneously with the antibodies indicated on the left following bead-manufacturers protocol. Dynabeads Protein A bind efficiently humanized Trastuzumab (II), while no direct binding of goat α-human Cy5 antibody occurs (III). Following pretreatment with the chimeric murine Cetuximab (IV) or Trastuzumab (not shown), the α-human antibody can be bound by the beads (IV, V).
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Heisig et al. BMC Microbiology 2011 11:163 doi:10.1186/1471-2180-11-163