Additional file 2.
Immunofluorescence microscopy showing the replication of Lm-spa+ in the cytosol of SK-BR-3 cells. SK-BR-3 cells were infected at a MOI 10 with L. monocytogenes strains ΔtrpS × pSP0-PactA-gfp (a), Lm-spa- × pSP0-PactA-gfp (b) and Lm-spa+ × pSP0-PactA-gfp (c) preincubated with 1 × PBS (i-iii) or Trastuzumab (iv-vi) and GFP-expression was monitored by fluorescence microscopy at the indicated time points. Bright field and fluorescence overlay images are shown. The L. monocytogenes control strain ΔtrpS × pSP0-PactA-gfp shows the typical intracellular life cycle independent of preincubation with Trastuzumab (a). L. monocytogenes strain Lm-spa- × pSP0-PactA-gfp is unable to infect SK-BR-3 cells as expected (b). L. monocytogenes strain Lm-spa+ × pSP0-PactA-gfp infects cells and replicates in the cytosol only after preincubation with Trastuzumab (c). Because of the aroA deletion Lm-spa+ × pSP0-PactA-gfp hardly spreads to neighboring cells.
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Heisig et al. BMC Microbiology 2011 11:163 doi:10.1186/1471-2180-11-163