Additional file 1.
Internalization of Cetuximab- or Trastuzumab- coated Lm-spa- relative to uncoated Lm-spa- (-mAb) into different cell lines. (a) Mouse mammary cancer cell line 4T1, the HER2 transduced isogenic 4T1-HER2 and (b) the human mammary/ovary cancer cell lines SK-BR-3 and SK-OV-3, respectively, were infected with a MOI of 100 with Lm-spa- after coating with different antibodies. Intracellular colony forming units (CFU) were determined after gentamicin treatment by serial plating and the internalization rate of the antibody-coated relative to the uncoated bacteria was calculated. As expected, the Lm-spa- strain (which is InlAB-negative) was not internalized by 4T1, 4T1-HER2, SKBR3 or SKOV3 cells regardless whether these bacteria were incubated with Cetuximab or Trastuzumab. Raw CFU data of intracellular bacteria used for calculation of (a) and (b) is shown in (c) and (d). Raw CFU data of intracellular bacteria used for calculation of Figure 2a and Figure 2b is shown in (e) and (f).
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Heisig et al. BMC Microbiology 2011 11:163 doi:10.1186/1471-2180-11-163