Effect of serum incubation on antibody-mediated internalization of Lm-spa+. The bacteria were incubated with PBS (-mAb), Cetuximab or Trastuzumab and the antibodies were covalently bound to protein A by crosslinking with DMP. Subsequently the bacteria were incubated with murine serum prior to infection of 4T1-HER2 cells. Intracellular CFU was determined after gentamicin treatment by plating serial dilutions. The relative internalization rate in comparison to uncoated bacteria was calculated and is shown.
Heisig et al. BMC Microbiology 2011 11:163 doi:10.1186/1471-2180-11-163