Internalization of Cetuximab- or Trastuzumab- coated Lm-spa+ relative to uncoated Lm-spa+ (-mAb) into different cell lines. (a) Mouse mammary cancer cell line 4T1, the HER2 transduced isogenic 4T1-HER2 and (b) the human mammary/ovary cancer cell lines SK-BR-3 and SK-OV-3, respectively, were infected with Lm-spa+ after coating with different antibodies. Intracellular colony forming units (CFU) were determined after gentamicin treatment by serial plating and the internalization rate of the antibody-coated relative to the uncoated bacteria was calculated.
Heisig et al. BMC Microbiology 2011 11:163 doi:10.1186/1471-2180-11-163