Effects of geldanamycin on growth and morphology. S. schenckii conidia (109) were inoculated in a modification of medium M containing 2, 5 and 10 μM concentrations of geldanamycin. The growth was recorded as OD at 600 nm at 3, 5 and 7 days of incubation as described in Methods. The percentage of growth of the S. schenckii in the presence of geldanamycin when compared to that of the controls of 3 independent experiments is given ± a standard deviation. Values significantly different from the controls are marked with an asterisk. Samples of the growth obtained after 7 days at 35°C in liquid medium w/wo geldanamycin (10 μM) were drawn and mounted on lactophenol cotton blue. Figure 6A corresponds to the controls cells at 40× magnification. Figure 6B shows the appearance of cells grown in the presence of geldanamycin at 20× magnification. Microscopic observations of the fungus were done using a Nikon Eclipse E600, equipped with a Nikon Digital Sight DS-2Mv and the NIS-Elements F 2.3 software.
Rodriguez-Caban et al. BMC Microbiology 2011 11:162 doi:10.1186/1471-2180-11-162