Table 1

Bacterial strains and plasmid used in this work

Strain

Relevant characteristics

Reference


Rhizobium etli CE3

Streptomycin resistant derivative of CFN42 strain

[20]

R. etli CFNX101

recA::Ω-Spectinomycin derivative of CE3

[46]

R. etli CFNX107

recA:: Ω-Spectinomycin derivative of CE3, laking plasmid p42a and p42d.

[46]

E. coli S17-1

Plasmid donor in conjugations

[23]

Plasmid

Relevant characteristics

Reference

pDOP

A chloramphenicol resistant suicide vector derived from pBC SK(+), and containing oriT

This work

pDOP-E'

pDOP derivative with the intergenic region repB-repC, the complete repC gene under Placpromoter, and 500 pb downstream repC stop codon.

[22]

pDOP-H3

pDOP derivative carrying a 5.6 Kb HindIII with repABC operon of R. etli plasmid p42d.

This work

pDOP-αC

pDOP derivative with the intergenic region repB-repC and the complete repC gene under Plac promoter.

This work

pDOP-C

pDOP carrying repC gen of plasmid p42d, with a SD sequence (AGGA) and under Plac promoter.

This work

pDOP-C/D1UM

Similar to pDOP-C but with a repC gene carrying a deletion from codon 2 to codon 29

This work

pDOP-C/RD1L

Similar to pDOP-C but with a repC gene carrying a deletion from codon 372 to codon 401

This work

pDOP-F1

pDOP containing a repC fragment from codon 2 to codon 110, with a SD consensus sequence under Plac promoter.

This work

pDOP-C/F1-F2

pDOP containing a repC fragment from codon 2 to codon 209, with a SD consensus sequence under Plac promoter.

This work

pDOP-C/F1-F3

pDOP containing a repC fragment from codon 2 to codon 309, with a SD consensus sequence under Plac promoter.

This work

pDOP-C/F4

pDOP containing a repC fragment from codon 310 to codon 403, with a SD consensus sequence under Plac promoter.

This work

pDOP-C/F4-F3

pDOP containing a repC fragment from codon 210 to codon 403, with a SD consensus sequence under Plac promoter.

This work

pDOP-C/F4-F2

pDOP containing a repC fragment from codon 111 to codon 403, with a SD consensus sequence under Plac promoter.

This work

pDOP-C s/SD

Similar to pDOP-C but without the SD sequence

This work

pDOP-TtMC

Similar to pDOP-C but with a mutant repC gene carrying

This work

silent mutations to increase its CG content

pDOP-CBbglll

Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the BglII restriction site

This work

pDOP-CSphI

Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the SphI restriction site

This work

pDOP-CAtLC

pDOP derivative carrying repC gen of the Agrobacterium

This work

tumefaciens C58 linear chromosome, with a SD sequence (AGGA) and under Plac promoter.

pDOP-CsA

pDOP derivative carrying repC gen of the Sinorhizobium meliloti 1021 pSymA, with a SD sequence (AGGA) and under Plac promoter.

This work

pDOP/C420-1209

pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the pSymA RepC protein and the rest of p42d.

This work

pDOP/C1-420

pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein and the rest of pSymA.

This work

pDOP/C421-840

pDOP with a hybrid repC gene encoding, the first 140 amino acid residues of the pSymA RepC protein, the next 140 amino acid residues from the p42d RepC protein and the rest from the pSymA RepC protein.

This work

pDOP/Cs421-840

pDOP witha hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein, the next 140 amino acid residues from the pSymA RepC protein and the rest from the p42d RepC protein.

This work

pDOP/C841-1209

pDOP derivative with a hybrid repC gene, encoding the first 280 aminoacid residued of pSymA RepC and the rest of p42d RepC protein.

This work

pDOP/C1-990

pDOP derivative with a hybrid repC gene, encoding the first 330 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein.

This work

pDOP/C1-1086

pDOP derivative with a hybrid repC gene, encoding the first 362 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein.

This work


Cervantes-Rivera et al. BMC Microbiology 2011 11:158   doi:10.1186/1471-2180-11-158

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