Strategy used to construct FMDV Asia1/JSp1c8 full-length cDNA clone, pRDD. The location of restriction enzyme cleavage sites used to assemble the subcloned RT-PCR fragments (E1, E2, E3, E4, E5 and E12) are shown (numbered relative to nucleotide position in the virus genome). Thick lines and an open box represent the untranslated regions and the open-reading frame for the viral polyprotein, respectively. The thin line represents the vector sequence. FMDV cDNA is under the control of the T7 promoter.
Li et al. BMC Microbiology 2011 11:154 doi:10.1186/1471-2180-11-154