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Open Access Highly Accessed Research article

Lactobacillus plantarum (VR1) isolated from an Ayurvedic medicine (Kutajarista) ameliorates in vitro cellular damage caused by Aeromonas veronii

Himanshu Kumar1, Ashraf Y Rangrez2, Kannayakanahalli M Dayananda3, Ashwini N Atre1, Milind S Patole1 and Yogesh S Shouche14*

Author Affiliations

1 Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind, Pune, Maharashtra, India

2 Department of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, F-80037, Amiens, France

3 Department of Chemical and Biological Engineering, State University of New York at Buffalo, Buffalo NY 14260, USA

4 Microbial Culture Collection, Hindustan Antibiotics Ltd. Complex, Pimpri, Pune 411 018, Maharashtra, India

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BMC Microbiology 2011, 11:152  doi:10.1186/1471-2180-11-152

Published: 27 June 2011

Abstract

Background

Lactobacillus plantarum is considered as a safe and effective probiotic microorganism. Among various sources of isolation, traditionally fermented foods are considered to be rich in Lactobacillus spp., which can be exploited for their probiotic attribute. Antibacterial property of L. plantarum has been demonstrated against various enteric pathogens in both in vitro and in vivo systems. This study was aimed at characterizing L. plantarum isolated from Kutajarista, an ayurvedic fermented biomedicine, and assessing its antagonistic property against a common enteropathogen Aeromonas veronii.

Results

We report the isolation of L. plantarum (VR1) from Kutajarista, and efficacy of its cell free supernatant (CFS) in amelioration of cytotoxicity caused by Aeromonas veronii. On the part of probiotic attributes, VR1 was tolerant to pH 2, 0.3% bile salts and simulated gastric juice. Additionally, VR1 also exhibited adhesive property to human intestinal HT-29 cell line. Furthermore, CFS of VR1 was antibacterial to enteric pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Aeromonas veronii and clinical isolates of P. aeruginosa and E. coli. Detailed study regarding the effect of VR1 CFS on A. veronii cytotoxicity showed a significant decrease in vacuole formation and detrimental cellular changes in Vero cells. On the other hand, A. veronii CFS caused disruption of tight junction proteins ZO-1 and actin in MDCK cell line, which was prevented by pre-incubation with CFS of VR1.

Conclusions

This is the first study to report isolation of L. plantarum (VR1) from Kutajarista and characterisation for its probiotic attributes. Our study demonstrates the antagonistic property of VR1 to A. veronii and effect of VR1 CFS in reduction of cellular damage caused by A. veronii in both Vero and MDCK cell lines.