Table 3

Genes expression regulated by saeRS in S. epidermidis

Genbank accession no.

Genes/ORF

Description

Expression ratio mutant/WT

P-valueb

Functions

References


Microarraya

RT-qPCR


Autolysis-related genes

AAW52842

lytS

two-component sensor histidine kinase LytS

3.87

2.33 ± 0.35

0.0097

Negatively modulating the expression of murein hydrolases and positively regulates the expression of the lrgAB operon in S. aureus

[27,43,44]

AAW52844

lrgA

holin-like protein LrgA

2.28

2.75 ± 0.05

< 0.0001

Encoding a murein hydrolase exporter similar to bacteriophage holin proteins; may be required for the activity or transport of this cell wall-associated murein hydrolase in S. aureus

[44]

AAW53428

serp0043

1,4-beta-N-acetylmuramidase

4.86

2.25 ± 0.20

0.0016

Having lysozyme activity in peptidoglycan catabolic process in S. aureus

[14]

AAW53918

glpQ

glycerophosphoryl diester phosphodiesterase GlpQ, putative

2.98

1.80 ± 0.20

0.0080

Having glycerophosphodiester phosphodiesterase activity in lipid and glycerol metabolic process in S. aureus

[55]

AAW54343

arlR

DNA-binding response regulator

8.30

3.20 ± 0.45

0.0015

Regulating extracellular proteolytic activity; may be involved in the modulation of expression of genes associated with growth and cell division; positively regulating a two-component system lytRS in S. aureus

[18,25,26,56-58]

AAW53968

atlE

S. epidermidis autolysin

UDc

1.45 ± 0.10

0.0053

Having amidase activity to cleave the amide bond between N-acetyl muramic acid and L-alanine; mediating lysis of a subpopulation of the bacteria and extracellular DNA release in S. epidermidis

[7,29,46]

AJ250905

aae

S. epidermidis autolysin/adhesin

UD

2.32 ± 0.38

0.0088

Having bacteriolytic activity and binding to fibrinogen, fibronectin and vitronectin in S. epidermidis

[8]

Biofilm-forming related genes

AAW53175

icaA

a gene of ica operon

UD

1.22 ± 0.13

0.20

Encoding N-acetyglucosaminyltransferase for synthesis of polysaccharide intercellular adhesin (PIA) which is important for biofilm formation of S. epidermidis

[2,31,59]

AAW53239

aap

accumulation-associated protein

UD

1.62 ± 0.06

0.0008

Contributing to intercellular adhesion and biofilm formation of S. epidermidis

[4,60,61]

sae operon

AAW53762

saeS

sensor histidine kinase SaeS

0.26

UD

Encoding a histidine kinase; involving in the tight temporal control of virulence factor expression in S. aureus

[18,47,62]

AAW53763

saeR

DNA-binding response regulator SaeR

0.14

UD

The response regulator SaeR binding to a direct repeat sequence in S. aureus; involving in anaerobic growth and nitrate utilization in S. epidermidis

[11,48]

AAW53764

saeQ

conserved hypothetical protein

UD

UD

Encoding a membrane protein, function unknown in S. epidermidis

[62]

AAW53765

saeP

lipoprotein, putative

UD

UD

Encoding a lipoprotein, function unknown in S. epidermidis

[62]


a The complete raw microarray dataset has been posted on the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/ webcite), accession number GPL13532 for the platform design and GSE29309 for the original dataset.

b P-values of RT-qPCR results were caculated using Student's t-test.

c UD: under detection level in microarray analysis or by RT-qPCR.

Lou et al. BMC Microbiology 2011 11:146   doi:10.1186/1471-2180-11-146

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