Table 1

Bacterial strains and plasmids used in the present study

Strain or plasmid

Relevant genotype or characteristic

Reference or source


Strains

E. coli DH5α

λ- ϕ80dlacΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK- mK-) supE44 thi-1 gyrA relA1

[49]

SE1457

Biofilm positive strain

[50]

S. aureus RN4220

Restriction-negative, modification-positive isolate

[51]

SE1457ΔsaeRS

saeRS deletion mutant of strain 1457, Spcr

This study

SE1457saec

1457ΔsaeRS complemented with saeRS

This study

Plasmids

pET-28a(+)

Expression vector, KanR

Novagen

pBT2

pCX19

Temperature-sensitive E. coli- Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus)

Derivate of pCX15

[52]

[53]

pMAD

Escherichia coli/Staphylococcus Shuttle vector

[54]

pMAD-saeRS

Vector for allelic gene replacement of saeRS in S. epidermidis

This study

pBT2-saeRS

Vector for complementation of saeRS in S. epidermidis 1457ΔsaeRS

This study


*Abbreviations: Amp, ampicillin; Cm, chloramphenicol; Em, erythromycin.

Lou et al. BMC Microbiology 2011 11:146   doi:10.1186/1471-2180-11-146

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