1D SDS - PAGE and Total Protein Concentration in BCM and PCM. The total protein concentration in BCM and PCM did not differ drastically (A), but several differences in the extracellular proteome of planktonic and biofilm cultures of S. aureus were revealed by 1D SDS-PAGE (B). The presence of a smear and low molecular weight peptides in the BCM indicates the presence of a bacterial protease. Bands in (B) marked with an arrow were excised and analyzed by HPLC-MS/MS (Table 1).
Secor et al. BMC Microbiology 2011 11:143 doi:10.1186/1471-2180-11-143