Table 2

Community composition based on CARD-FISH analysis

Samples

% of cell count 1

% of aggregate count 1

% of biovolume1


S1

ANME-1

Below detection limit2

Below detection limit2

Below detection limit2

ANME-2

8.2 ± 3.0

37.1 ± 6.2

13.4 ± 4.2

ANME-3

0.1 ± 0.1

2.1 ± 1.4

1.5 ± 1.5

SRB

2.9 ± 1.5

32.0 ± 6.2

22.7 ± 5.3


S2

ANME-1

Below detection limit3

Below detection limit3

Below detection limit3

ANME-2

2.5 ± 2.0

47.2 ± 8.2

50.4 ± 15.9

ANME-3

0.1 ± 0.1

0.8 ± 0.7

2.4 ± 1.8

SRB

0.8 ± 0.4

37.6 ± 5.0

60.6 ± 5.5


1 The average value and standard error were calculated based on 50 fields of view on each hybridization. No ANME-1 cell or aggregate was observed based on our method. 2 Detection limit of 4 × 104 cells/ml slurry. 3 Detection limit of 9 × 104 cells/ml slurry

Zhang et al. BMC Microbiology 2011 11:137   doi:10.1186/1471-2180-11-137

Open Data