Table 1

Primers and probes used in this study.

Name (labelling)

Sequence (5' to 3')

Positions

Specificity

References


PCR primers

Arch-21f

TTC CGG TTG ATC CYG CCG GA

21-40

Archaea

[28]

Arch-958r

YCC GGC GTT GAM TCC AAT T

958-976

Archaea

[28]

27f

AGA GTT TGA TCC TGG CTC AG

27-46

Eubacteria

[29]

1492r

GGT TAC CTT GTT ACG ACT T

1492-1510

Eubacteria

[30]


CARD-FISH probes

ANME1-350

AGT TTT CGC GCC TGA TGC

350-367

ANME-1 archaea

[4]

EelMS932

AGC TCC ACC CGT TGT AGT

932-949

ANME-2 archaea

[4]

ANME3-1249

TCG GAG TAG GGA CCC ATT

1250-1267

ANME-3 archaea

[31]

ANME3-1249H3

GTC CCA ATC ATT GTA GCC GGC

1229-1249

Helper probe for ANME3-1249

[32]

ANME3-1249H5

TTA TGA GAT TAC CAT CTC CTT

1268-1288

Helper probe for ANME3-1249

[32]

DSS658

TCC ACT TCC CTC TCC CAT

658-685

Desulfosarcina spp., Desulfofaba spp., Desulfococcus spp., Desulfofrigus spp.

[33]


Zhang et al. BMC Microbiology 2011 11:137   doi:10.1186/1471-2180-11-137

Open Data