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Open Access Research article

Enrichment of a microbial community performing anaerobic oxidation of methane in a continuous high-pressure bioreactor

Yu Zhang12, Loïs Maignien2, Xianxian Zhao1, Fengping Wang1 and Nico Boon2*

Author Affiliations

1 State Key Laboratory of Microbial Metabolism and School of Life Science & Biotechnology, State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Dongchuan Rd. 800, Shanghai, 200240, P. R. China

2 Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, 9000, Belgium

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BMC Microbiology 2011, 11:137  doi:10.1186/1471-2180-11-137

Published: 16 June 2011

Abstract

Background

Anaerobic oxidation of methane coupled to sulphate reduction (SR-AOM) prevents more than 90% of the oceanic methane emission to the atmosphere. In a previous study, we demonstrated that the high methane pressure (1, 4.5, and 8 MPa) stimulated in vitro SR-AOM activity. However, the information on the effect of high-pressure on the microbial community structure and architecture was still lacking.

Results

In this study we analysed the long-term enrichment (286 days) of this microbial community, which was mediating SR-AOM in a continuous high-pressure bioreactor. 99.7% of the total biovolume represented cells in the form of small aggregates (diameter less then 15 μm). An increase of the total biovolume was observed (2.5 times). After 286 days, the ANME-2 (anaerobic methanotrophic archaea subgroup 2) and SRB (sulphate reducing bacteria) increased with a factor 12.5 and 8.4, respectively.

Conclusion

This paper reports a net biomass growth of communities involved in SR-AOM, incubated at high-pressure.