Growth and stationary-phase survival under low salinity. Cells were grown in 0.1% NaCl LB. A: Growth was monitored by optical density (OD) measurements. N315: filled diamonds; cls1 mutant: filled squares; cls2 mutant: filled triangles; cls1/cls2 double mutant: open circles. Optical densities were checked at least twice, and the means are shown. After 47 h, the OD of only N315 and its cls1/cls2 double mutant were measured. B: Number of CFUs during the long incubation. The means and standard deviations of at least three independent experiments are shown. C: Thin-layer chromatography of phospholipids. Cells were harvested at 23 h. The phospholipid profile was confirmed to be similar up to 153 h (data not shown). The means and standard deviations of relative signal intensities from two independent experiments are shown on the right.
Tsai et al. BMC Microbiology 2011 11:13 doi:10.1186/1471-2180-11-13