Open Access Highly Accessed Research article

Staphylococcus aureus requires cardiolipin for survival under conditions of high salinity

Melody Tsai1, Ryosuke L Ohniwa1, Yusuke Kato2, Sayaka L Takeshita1, Toshiko Ohta1, Shinji Saito1, Hideo Hayashi3 and Kazuya Morikawa1*

Author Affiliations

1 Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8575, Japan

2 Division of Insect Sciences, National Institute of Agrobiological Sciences, Oowashi 1-2, Tsukuba 305-8634, Japan

3 Chugoku Gakuen University, Niwase 83, Kitaku, Okayama 701-0197, Japan

For all author emails, please log on.

BMC Microbiology 2011, 11:13  doi:10.1186/1471-2180-11-13

Published: 18 January 2011

Additional files

Additional file:

Figure S1 - The phospholipid analysis of ASABF-α-susceptible strains and resistant strains. Strains N315, NKSB, NKSBv, and MRSA no. 33 are susceptible to ASABF-α, and strains NKSBm, MRSA no. 7, and Mu50 are resistant [33]. Cells were harvested at stationary phase. Lipids were extracted by the chloroform-methanol method without (A) or with (B) the lysostaphin treatment. Solvent system: chloroform-methanol-acetic acid (65:25:10; v/v/v). Mu50 has unusually thick cell walls (ref*) and required higher lysostaphin concentration for efficient CL extraction (data not shown). ref*: Cui, L., X. Ma, K. Sato, K. Okuma, F. C. Tenover, E. M. Mamizuka, C. G. Gemmell, M. N. Kim, M. C. Ploy, N. El-Solh, V. Ferraz, and K. Hiramatsu. 2003. Cell wall thickening is a common feature of vancomycin resistance in Staphylococcus aureus. J Clin Microbiol 41:5-14.

Format: PDF Size: 1.3MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data