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Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin

Cláudio Pereira Figueira1, Julio Croda16, Henry A Choy23, David A Haake23, Mitermayer G Reis1, Albert I Ko14 and Mathieu Picardeau5*

Author Affiliations

1 Oswaldo Cruz Foundation, Brazilian Ministry of Health, Gonçalo Moniz Research Center, Rua Waldemar Falcão, 121, 40295-001 Salvador, Bahia, Brazil

2 Veterans Affairs Greater Los Angeles Health Care System, Division of Infectious Diseases, 111F, 11301 Wilshire Blvd, Los Angeles, CA 90073, California, USA

3 Department of Medicine, University of California Los Angeles School of Medicine, Los Angeles, California, USA

4 Division of Epidemiology of Microbial Diseases, Department of Epidemiology and Public Health, Yale University School of Medicine, Division of Epidemiology of Microbial Disease, 319 LEPH - 60 College St., New Haven, CT 06510 New Haven, USA

5 Institut Pasteur, Unité de Biologie des Spirochètes, 28 rue du docteur Roux, 75724 Paris Cedex 15, France

6 Faculty of Health Sciences, Federal University of Grande Dourados, Brazil

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BMC Microbiology 2011, 11:129  doi:10.1186/1471-2180-11-129

Published: 9 June 2011

Additional files

Additional file 1:

surface immunofluorescence assays in L. interrogans. Immunofluorescence assays were performed with L. interrogans strain Fiocruz L1-130, which was labeled with normal rabbit serum (control) and antibodies against LigA (LigANI), LigB (LigBNI), GroEL, and LPS. Alexa- and fluorescein isothiocyanate-conjugated secondary antibodies were used to detect surface-bound antibodies. A DAPI counterstain was used to document the presence of leptospires. The photomicrograph show the results of one of three representative experiments.

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