Most of the identified C. pseudotuberculosis exoproteins were predicted by the SurfG+ program as having an extracytoplasmic localization. The proteins identified in the exoproteomes of each C. pseudotuberculosis strain were analyzed by SurfG+ and attributed a probable final sub-cellular localization. Proteins classified as having a cytoplasmic localization were further analyzed with the SecretomeP tool for prediction of non-classical (leaderless) secretion. Besides, literature evidence for exportation by non-classical pathways was also used to re-classify the cytoplasmic proteins (see text for details). SE = secreted; PSE = potentially surface exposed; C = cytoplasmic; M = membrane; NCS = non-classically secreted.
Pacheco et al. BMC Microbiology 2011 11:12 doi:10.1186/1471-2180-11-12