Open Access Highly Accessed Research article

A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis

Luis GC Pacheco123, Susan E Slade4, Núbia Seyffert2, Anderson R Santos2, Thiago LP Castro2, Wanderson M Silva2, Agenor V Santos1, Simone G Santos5, Luiz M Farias5, Maria AR Carvalho5, Adriano MC Pimenta1, Roberto Meyer3, Artur Silva6, James H Scrivens4, Sérgio C Oliveira1, Anderson Miyoshi2, Christopher G Dowson4 and Vasco Azevedo2*

Author Affiliations

1 Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Belo Horizonte, 31.270-901, Brazil

2 Department of General Biology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Belo Horizonte, 31.270-901, Brazil

3 Institute of Health Sciences, Universidade Federal da Bahia, Av. Reitor Miguel Calmon, Salvador, 40.110-902, Brazil

4 School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, United Kingdom

5 Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Belo Horizonte, 31.270-901, Brazil

6 Genome and Proteome Network of the State of Pará, Universidade Federal do Pará, R. Augusto Corrêa, Belém, 66.075-110, Brazil

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BMC Microbiology 2011, 11:12  doi:10.1186/1471-2180-11-12

Published: 17 January 2011

Additional files

Additional file 1:

Figure S1. Comparison between the experimental (A) and virtual (B) 2-D gels of the exoproteome of the strain 1002 of C. pseudotuberculosis. (A) 2D-gel with 150 μg of TPP extracted extracellular proteins of the 1002 strain. Proteins were separated in the first dimension by isoelectric focusing using strips of 3.0-5.6 NL pI range (GE Healthcare). Visualization was by Colloidal Coomassie staining. (B) The virtual 2D-gel was generated with the theoretical pI and MW values of the proteins identified by LC-MSE.

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Additional file 2:

Table S1. Proteins composing the core C. pseudotuberculosis exoproteome, identified by LC-MSE.

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Additional file 3:

Table S2. Variant exoproteome of the strain 1002 of Corynebacterium pseudotuberculosis.

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Additional file 4:

Table S3. Variant exoproteome of the strain C231 of Corynebacterium pseudotuberculosis.

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Additional file 5:

Figure S2. Predictions of LPXTG motif-containing proteins, lipoproteins and Tat-pathway associated signal peptides in the exoproteomes of the strains 1002 and C231 of C. pseudotuberculosis.

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Additional file 6:

Figure S4. A conserved hypothetical exported protein present in the Genome of the strain C231 but absent from the strain 1002 of C. pseudotuberculosis. The two sequenced Genomes were aligned using the Artemis Comparison Tool (ACT). The arrows point to tRNA genes.

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Additional file 7:

Table S4. Relative expression analysis of the extracellular proteins common to the strains 1002 and C231 of Corynebacterium pseudotuberculosis.

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Additional file 8:

Figure S5. Distribution of orthologous proteins of the C. pseudotuberculosis experimental exoproteins throughout other experimentally confirmed exoproteomes of pathogenic corynebacteria, as determined through transitivity clustering analysis. The 19 C. pseudotuberculosis exoproteins only identified in the exoproteomes of other pathogenic corynebacteria are presented in the table. Cp = C. pseudotuberculosis; Cd = C. diphtheriae; Cj = C. jeikeium.

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Additional file 9:

Supplementary information on the bioinformatics tools used in this study.

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