Table 2

SPR analysis of His6-polypeptides

Polypeptide

KD to Fn (M) *

KD to Fg (M) *


His-ΔNarG

0,77

0,72

His-ΔFnBPA

5,24 × 10-6

0,31

His-ΔEbh

0,02

1,25 × 10-6

His-ΔCoa

< 0

1,80 × 10-7

His-ΔPurK

4,43 × 10-7

5,39 × 10-6

His-ΔUsp

0,35

6,45 × 10-6

His-ΔPBP

0,36

0,13


* the steady state affinity constants (KD) of the seven analytes tested are shown in molar concentrations; values shown in bold indicate high affinity for the indicated ligand (Fn or Fg).

† affinity was not measurable since all values were negative

Kylväjä et al. BMC Microbiology 2011 11:117   doi:10.1186/1471-2180-11-117

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