Table 1

Examples of Ftp library clones that express adhesive polypeptides

Clone Name

Length of insert*

Chromosomal location of insert

ORFsin insert

Predicted gene product(s) of the Ftp-clone

Presence of FliC1-20 and/or FLAG-tag in the gene product

Binding specificity of the product

Predicted molecular mass#


ΔNarG

393

2465481-2465873

1) 02681

NarG§1

FliC1-20

FLAG-tag

None

18.5

ΔFnBPA

346

2581863-2582208

1) 02803

FnBPA§2

FliC1-20

FLAG-tag

Fn

16.6

ΔEbh

582

1398633-1399214

1) 01447

Ebh§2

FliC1-20

FLAG-tag

Fn

24.2

ΔCoa

825

212434-213258

1) 00192

coagulase

FliC1-20

FLAG-tag

Fg, Fn

34.2

ΔPurK

383

979768-980150

1) 01008

out of frame

No

2) 01009

PurK §1

FLAG-tag

Fn, Fg

14.6

ΔSCOR

484

2667518-2668001

1) 02897

terminator in sequence

FliC

2) 02898

Putative SCOR §1

FLAG-tag

Fn, Fg

17.7

ΔUsp

664

1724620-1725283

1) 01818

out of frame

No

2) 01819

Usp§1 -like

FLAG-tag

Fn, Fg, CIV,

19.3

ΔIspD

885

244692-245576

1) 00223

out of frame

No

2) 00225

IspD§2

FLAG-tag

Fn, Fg

13.4

ΔPBP

756

2257336-2258091

1) 02433

out of frame

No

2) 02432

out of frame

No

3) 02430

putative PBP§1

of ABC§1transporter

FLAG-tag

Fn, Fg

6.7


* In base pairs

† In S. aureus subsp. aureus NCTC 8325

‡ Open reading frames (ORFs) in the clones are partial, the number refers to the systematic gene identifier SAOUHSC_no. in the GenBank database, a locus_tag

§1 Abbreviations of TIGR Family names: NarG, nitrate reductase α-subunit; PurK, Phosphoribosylamino-imidazole carboxylase ATPase subunit; SCOR, short-chain oxidoreductase; Usp, universal stress protein family; PBP, periplasmic binding protein; ABC, ATP-binding cassette

§2 Abbreviations of the protein names: FnBPA, fibronectin binding protein A; Ebh, extracellular matrix binding protein homologue; IspD, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

The reading frame is in relation to fliC and flag sequences

# Molecular mass in kilodaltons. The molecular mass of FliC1-20 and FLAG-tag included when present in the gene product

Kylväjä et al. BMC Microbiology 2011 11:117   doi:10.1186/1471-2180-11-117

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