Dynamic range and sensitivity of the Campylobacter coli and Campylobacter jejuni real-time PCR assays for faecal samples. Standard curves of DNA extracted from the Campylobacter-negative faecal samples spiked with 10-fold serial dilution of (a) C. coli CIP 70.81 suspension, ranging 2.5 × 102 to 2.5 × 107 CFU/g of faeces and (b) C. jejuni NCTC 11168 suspension, ranging 2.0 × 102 to 2.0 × 107 CFU/g of faeces, each dot representing the result of duplicate amplification of each dilution. The coefficients of determination R2 and the slopes of the regression curve are indicated. The standard curve is obtained by correlation of the threshold cycle values (Ct) and log10 input CFU/g of faeces (Log CO) from the amplification plot.
Leblanc-Maridor et al. BMC Microbiology 2011 11:113 doi:10.1186/1471-2180-11-113