Correlating HOCl-induced ATP changes with bacterial viability. ATP production is affected by HOCl exposure and correlates statistically with CFU viability in PsA, BC, and EC (p = 0.005, 0.006, and 0.01, respectively); however, SA and KP lose CFU viability after exposure to lower concentrations of HOCl than are required to abolish ATP production during the assay time. Solid circles and lines: ATP recovery after oxidant exposure. Open circles and dotted lines: CFU viability. Both parameters are measured as percent relative to oxidant-free controls. P-values represent linear regression of the raw data values from percent ATP recovery versus CFU viability. Values less than 0.05 were considered significant and denote correlation among the parameters; values greater than 0.05 indicate independence of the parameters. Error bars represent standard deviation of at least n = 3 experiments.
Bonvillain et al. BMC Microbiology 2011 11:112 doi:10.1186/1471-2180-11-112