Bacterial killing by reagent H2O2 and HOCl in vitro. Microbes were exposed to various concentrations of H2O2 or HOCl, as indicated, for 1 hour at 37°C. At the end of the exposure, the samples were plated to LB agar plates for overnight culture. Bacterial killing by oxidants was measured as percent of viable bacteria relative to the number of colonies from the oxidant-free controls. A) Organisms indicated were exposed to 0 mM to 5.0 mM H2O2 or (B) 0 mM to 0.1 mM HOCl. PsA = P. aeruginosa, SA = S. aureus, BC = B. cepacia, KP = K. pneumoniae, and EC = DH5α-E. coli. Error bars represent standard deviation of at least n = 3 experiments.
Bonvillain et al. BMC Microbiology 2011 11:112 doi:10.1186/1471-2180-11-112