Profile of M-cadherin mRNA expression by SkMC experimentally infected with T. gondii. (A) The arbitrary values presented in the graph are based on the densytometric analysis of the PCR gel image shown in panel B, corresponding to 3, 12 and 24 h of infection. Light bars indicate uninfected control cells and black bars indicate the infected cells. (B) Polyacrylamide, silver stained gels for visualization of the amplified M-cadherin and GAPDH mRNAs (from top to bottom, respectively). Lanes 1, 3 and 5 show the profiles of negative controls and lanes 2, 4 and 6 the profiles of infected cells (3, 12 and 24 h, respectively). NC, negative PCR control. Molecular size markers are indicated to the left. Student's T-test (*) p ≤ 0.05.
Gomes et al. BMC Microbiology 2011 11:110 doi:10.1186/1471-2180-11-110