Table 1

The strains and plasmids utilized in this work

Bacterial strains

Genotype

Reference


Escherichia coli XL1-Blue

recA1, endA1, gyrA96, thi-1, hsdR17(rK-, mK+), supE44, relA1, lac, [F', proAB, lacIqZΔM15::Tn10(tetr)]

Stratagene


Azospirillum amazonense Y2

wild type

EMBRAPA-RJ


Azospirillum amazonense delK

Y2 derivative, ΔglnK

This work


Plasmids

Relevant characteristics

Reference

Purpose


pUC18

cloning vector, ampr, lacZα

Clontech

cloning procedures


pAAGLNK

pUC18 derivative containing the SalI genomic fragment of the glnK region

This work

glnK isolation


pGLNBA

pUC18 derivative containing the amplification product generated from the revsf_glmBint and gln_AA_do primers

This work

glnB-glnA intergenic region and partial glnA isolation


pRK2013

ColE1 ori, kanr, mob, tra

[45]

helper plasmid for conjugation experiments


pHRGFPGUS

pBBR1 ori, kanr, mob, gfpmut3

[46]

DNA transfer evaluation, reporter vectors construction


pPZP201BK

pVS1 ori, ColE1 ori, kanr, mob

[47]

construction of pPZPLACEYFP


pEYFP

pUC ori, eyfp, ampr

Clontech

contruction of reporter plasmids


pPZPLACEYFP

PvuII-EcoRI fragment from pEYFP (containing the lac promoter-eyfp gene fusion) cloned into the EcoRV-EcoRI sites of pPZP201BK

This work

DNA transfer evaluation, positive control in the reporter assay


pK19MOBSACB

Integration vector, kanr, ColE1 replication origin, mob, sacB, lacZα

[48]

glnK mutagenesis


pKΔK

pK19MOBSACB derivative containing the flanking regions of the glnK gene joined by Crossover PCR

This work

glnK mutagenesis


pAATEYFP

pEYFP derivative containing the BglII-NcoI fragment of pAAGLNK cloned into its NcoI-BamHI sites

This work

construction of pHRAATEYFP


pPBEYFP

pEYFP derivative containing the intergenic region between the bcr protein superfamily gene and the glnB gene cloned into its NcoI-BamHI sites

This work

construction of pHRPBEYFP


pPKEYFP

pEYFP derivative containing the intergenic region between the ubiH and the glnK genes cloned into its NcoI-BamHI sites

This work

construction of pHRPKEYFP


pHRAATEYFP

HindIII-EcoRI fragment from pAATEYFP (containing the aat promoter-eyfp gene fusion) joined with the 5.8 kb HindIII-EcoRI fragment of pHRGFPGUS

This work

promoter evaluation


pHRLACEYFP

PvuII-EcoRI fragment from pEYFP (containing the lac promoter-eyfp gene fusion) joined with the 7.4 kb EcoRV-EcoRI fragment of pHRGFPGUS

This work

promoter evaluation


pHRPBEYFP

HindIII-EcoRI fragment from pPBEYFP (containing the glnB promoter-eyfp gene fusion) joined with the 5.8 kb HindIII-EcoRI fragment of pHRGFPGUS

This work

promoter evaluation


pHRPKEYFP

HindIII-EcoRI fragment from pPKEYFP (containing the glnK promoter-eyfp gene fusion) joined with the 5.8 kb HindIII-EcoRI fragment of pHRGFPGUS

This work

promoter evaluation


pHREYFP

HindIII-EcoRI fragment from pEYFP (containing the eyfp gene) joined with the 5.8 kb HindIII-EcoRI fragment of pHRGFPGUS

This work

promoter evaluation


Sant'Anna et al. BMC Microbiology 2011 11:107   doi:10.1186/1471-2180-11-107

Open Data