Electrical parameters tested for the A. amazonense electroporation. A - Effect of electrical field strength on the transformation efficiency of A. amazonense. Competent cells were electroporated at the electric field strengths indicated with the pHRGFPGUS vector, with the GenePulser apparatus set at 200 Ω and 25 μF. B - Effect of the pulse length on the transformation efficiency of A. amazonense. Competent cells were electroporated with different pulse lengths, using 50 ng of the pHRGFPGUS vector and with the GenePulser apparatus set at 12.5 kV/cm and 25 μF. The pulse lengths 2.2 ms, 4.3 ms, 7.3 ms and 10.5 ms are obtained setting the GenePulser apparatus at 100 Ω, 200 Ω, 400 Ω and 600 Ω, respectively.
Sant'Anna et al. BMC Microbiology 2011 11:107 doi:10.1186/1471-2180-11-107