Table 1

Bacterial strains and plasmids

Strains

genotype

origin


E. coli

DH5α

endA-1 hsdR-17 supE-44 thi-1 recA-1 gyrA relA-1 Δ(lacZYA-argG)U169 deoR

[57]

MT616

MM294 pRK600 CmR

[58]

BL21(DE3)

F- dcm ompT hsd gal/λ(DE3)

[59]

Sinorhizobium meliloti

Rm1021

SU47 SmR

[60]

R6.48

Rm1021,ohrR::GmR

This study

R7.15

Rm1021,ΔohrR ohr::GmR

This study

R7.16

Rm1021,ohr+ ohrR+ ohr::lacZ ohrR::uidA

This study

R8.39

Rm1021,ohr::GmR

This study

Plasmids

pGEMT

pUC derivative cloning vector, AmpR

Promega

pGEMTeasy

pUC derivative cloning vector, AmpR

Promega

pET22b+

expression vector, AmpR

Novagen

pK18mobsacB

mobilisable pUC derivative, sacB NeoR

[51]

pBBR1-MCS2

broad host range replicating mobilisable vector, NeoR

[61]

pBBR1-MCS5

broad host range replicating mobilisable vector, GmR

[61]

pTH1505

GmR, gfp, lacZ, uidA, rfp fusion vector

[54]

p34SGm

ori ColEI AmpR GmRcassette

[52]

pD3001

pK18mobsacB (XbaI-PstI)/ohrR downstream region (XbaI-NsiI)

this study

pD3083

pGEMTeasy/ ohrR upstream region

This study

pD4116

pK18mobsacB ΔohrR

This study

pD4244

pK18mobsacB ΔohrR::GmR

This study

pD5333

pK18mobsacBΔohrR ohr::GmR

This study

pD5455

pTH1505 ohr::lacZ, ohrR::uidA

This study

pD8657

pK18mobsacB ohr::GmR

This study

pBBohr

pBBRI-MCS2 ohr+

This study

pBBohrR

pBBRI-MCS2 ohrR+

This study

pE1541

pBBRI-MCS2 ohr::lacZ

This study

pETohrR

pET22b+ ohr+

This study


Fontenelle et al. BMC Microbiology 2011 11:100   doi:10.1186/1471-2180-11-100

Open Data