Oxidation promotes OhrR dimerisation and inactivation. (A) OhrR purified protein (20 nmoles) was incubated for 15 min with CuOOH (0.55 nM ) or H2O2 (0.5 nM ) and then, when indicated, added with 0.5 mM DTT and incubated for another 15 min. (B) The DNA fragment (20 pmoles) corresponding to ohr-ohrR intergenic region was incubated with purified OhrR protein (20, 50 or 100 pmoles) in the presence of 0.5 nM H2O2 and in the absence or in the presence of 0.5 mM DTT.
Fontenelle et al. BMC Microbiology 2011 11:100 doi:10.1186/1471-2180-11-100