Table 2

Identification of non-assigned TTGE bands by excision, cloning and sequencing

Band Designation1

Bacterial species

Accession number2

Similarity (%)


c

Corynebacterium variabile

GenBank:AJ783438

98.3

f 3

uncultured bacterium from marine sediment

GenBank:FJ717185

97.2

m

Brachybacterium paraconglomeratum

GenBank:AJ415377

96.8

x

Agrococcus casei

GenBank:DQ168427

100

y

Alkalibacterium kapii

GenBank:AB294171

97.5


1 These designations are used to annotate bands from TTGE gels in figures 2 and 3.

2 Closest 16S rDNA sequence in the GenBank public database http://www.ncbi.nlm.nih.gov webcite.

3The 16S rDNA sequence of band f exhibited highest similarity of 94% with Clostridiisalibacter paucivorans (GenBank: EF026082), a bacterium that belong to cluster XII of the Clostridium subphylum [53].

Roth et al. BMC Microbiology 2010 10:74   doi:10.1186/1471-2180-10-74

Open Data