Table 1

Bacterial composition of cheese surface consortium F by a culture dependent method1

Bacterial species

Accession number2

Similarity (%)

Isolation media3

Viable count [CFU cm-2]

Percentage on TGYA


Brevibacterium linens

(or Brevibacterium aurantiacum4)

GenBank:AJ315491

(GenBank:X765664)

95.5-98.0

(97.8)

TGYA

7.5.108

32.5%

Staphylococcus vitulinus

GenBank:NR_024670

99.6

TGYA

6.0.108

26.0%

Brachybacterium tyrofermentans

GenBank:X91657

97.9

TGYA

4.5.108

19.5%

Corynebacterium casei

GenBank:DQ361013

100.0

TGYA

1.5.108

6.5%

Microbacterium gubbeenense

GenBank:AF263564

97.9

TGYA

1.5.108

6.5%

Marinilactibacillus psychrotolerans

GenBank:AB083413

99.8

TGYA

1.5.108

6.5%

Brachybacterium sp.

GenBank:AF513397

99.9

TGYA

0.7.108

3.0%


Staphylococcus equorum

GenBank:NR_027520

98.8-99.1

MSA

3.0.108

-

Staphylococcus epidermidis

GenBank:NC_004461

98.5

MSA

8.107

-


Facklamia tabacinasalis

GenBank:Y17820

99.1

BP

6.105

-


Lactococcus lactis

GenBank:NC_002662

99.5

MRS

4.104

-

Enterococcus devriesei

GenBank:AJ891167

98.2

MRS

1.104

-

Enterococcus malodoratus

GenBank:Y18339

99.8

MRS

2.103

-


Enterococcus faecalis

GenBank:AJ420803

99.3

KFS

2.102

-

Enterococcus faecium

GenBank:EU547780

100.0

KFS

6.101

-


1 128 isolates, i.e. ca. 25 isolates per media, were analyzed by TTGE and grouped into identical TTGE profiles. A representative isolate of each profile was identified by 16S rDNA sequencing. After the assignment of all isolates to a species, the percentage of each species on each of the five media was assessed. The cell count of a species was calculated by multiplying percentage and cell count determined for the corresponding media.

2 Closest 16S rDNA sequence in the GenBank public database http://www.ncbi.nlm.nih.gov webcite.

3 Total cell count was determined on TGYA. In addition, staphylococci were enumerated on BP agar and MSA, lactic acid bacteria on MRS agar and enterococci on KFS agar.

4 Given the polymorphy in the intraspecies diversity of B. linens (Oberreuter et al. [52]), strain assignation to B. linens or the related species B. aurantiacum based on 16S rDNA analysis only was considered not reliable.

Roth et al. BMC Microbiology 2010 10:74   doi:10.1186/1471-2180-10-74

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