Table 1

Bacterial strains and plasmids.

Strain/Plasmid

Relevant characteristics

Reference/Source


Bacteria

S. meliloti

1021

Wild-type SU47 derivative, Smr

[75]

2011

Wild-type SU47 derivative, Smr

[76]

1021Δhfq

1021 hfq mutant strain, Smr

This work

2011-1.2

2011 hfq insertion derivative (control str.), Smr, Kmr

This work

2011-3.4

2011 hfq insertion mutant, Smr, Kmr

This work

1021hfqFLAG

1021 derivative expressing a 3 × Flag-tagged Hfq, Smr

This work

E. coli

DH5α

F- endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG, φ80d, lacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ-

Bethesda Research Lab.

Plasmids

pRK2013

Helper plasmid, ColE1, Kmr

[77]

pGEM®-T Easy

Cloning vector for PCR, Apr

Promega Corporation

pK18mobsacB

Suicide vector in S. meliloti Kmr, sacB, oriV

[78]

pJB3Tc19

Broad host-range IncP cloning vector, Apr, Tcr

[79]

pBluescriptII KS+

Multicopy cloning vector, Apr

Stratagene

pGEMhfq

1,684-bp of hfq genomic region in pGEM-T

This work

pK18_1.2

Internal fragment of hfq ORF in pK18mobsacB

This work

pK18_3.4

Internal fragment of hfq ORF in pK18mobsacB

This work

pGEMΔhfq

Genomic region with deletion of hfq in pGEM-T

This work

pK18Δhfq

Genomic region with deletion of hfq in pK18

This work

pGEMHfq

pGEM-T containing hfq promoter and ORF

This work

pJBHfq

pJB3Tc20 containing hfq promoter and ORF

This work

pKS3 × Flag

3 × FLAG encoding fragment in pBluescriptII KS+

This work

pKS3 × Flag5

pKS3 × Flag with upstream region of hfq

This work

pKSHfq3 × Flag

pBluescriptII KS+ with Flag-tagged hfq ORF

This work

pGEMHfq3 × Flag

pGEM-T with Flag-tagged hfq ORF

This work

pK18Hfq3 × Flag

pK18mobsacB with Flag-tagged hfq ORF

This work


Torres-Quesada et al. BMC Microbiology 2010 10:71   doi:10.1186/1471-2180-10-71

Open Data