Binding of S. meliloti sRNAs to a FLAG-epitope tagged Hfq protein. Western-blot showing the specific recognition of the chromosomally encoded 3 × FLAG tagged Hfq protein by ANTI-FLAG M2® monoclonal antibodies in total protein extracts of two independent 1021hfqFLAG strains (i.e. two different clones arising from the second cross-over event) (left panel); and Northern analysis of CoIP RNA from the 1021hfqFLAG and wild-type strains for the detection of the Smr sRNAs (right panel). Lane 1 shows the expression pattern of the corresponding sRNAs in the wild-type strain.
Torres-Quesada et al. BMC Microbiology 2010 10:71 doi:10.1186/1471-2180-10-71