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Filifactor alocis - involvement in periodontal biofilms

Sebastian Schlafer1, Birgit Riep2, Ann L Griffen3, Annett Petrich1, Julia Hübner1, Moritz Berning1, Anton Friedmann2, Ulf B Göbel1 and Annette Moter1*

Author Affiliations

1 Institut für Mikrobiologie und Hygiene, Charité - Universitätsmedizin Berlin, Dorotheenstraße 96, 10117 Berlin, Germany

2 Abteilung Parodontologie, Centrum für Zahn-, Mund- und Kieferheilkunde, Charité - Universitätsmedizin Berlin, Aßmannhauser Straße 4-6, 14197 Berlin, Germany

3 Section of Pediatric Dentistry, College of Dentistry, Ohio State University, 305 W. 12th Avenue, Columbus, Ohio 43210, USA

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BMC Microbiology 2010, 10:66  doi:10.1186/1471-2180-10-66

Published: 1 March 2010

Additional files

Additional file 1:

Optimization of probe FIAL for FISH using the program daime. FISH was performed incubating fixed cells of F. alocis and F. villosus with different hybridization mixes. Signal intensities (Relative fluorescent Units, RU) emitted by F. alocis and F. villosus at different formamide concentrations were calculated from images taken with a fixed exposure time. Due to unspecific binding of FIAL, the light emission of F. villosus cells remained below 50 RU at every level of formamide. The signal emitted by F. alocis cells was considered sufficient using formamide concentrations of up to 20% (v/v).

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