Table 4

Phosphatases in cell extracts of the cysQ mutants

Substrate

H37Rv

ΔcysQ 203/12

ΔcysQ203/16


Fructose-1,6-bisP

18.94 ± 1.00 (6)

13.09 ± 1.24 (6)

12.41 ± 0.54 (7)


Inositol-1-P

0.40 ± 0.09 (8)

0.49 ± 0.17 (9)

0.57 ± 0.16 (9)


Inositol-2-P

0.84 ± 0.12 (8)

0.90 ± 0.27 (10)

0.70 ± 0.23 (10)


Glycerol-2-P

0.75 ± 0.32 (8)

1.02 ± 0.27 (10)

0.55 ± 0.15 (10)


Glycerol-3-P

-0.37 ± 0.28 (3)

-0.35 ± 0.14 (3)

0.27 ± 0.45 (3)


5' AMP

1.42 ± 0.31 (3)

1.69 ± 0.14 (3)

1.39 ± 0.03 (3)


p-nitrophenyl-P

5.51 ± 0.36 (2)

3.64 ± 1.92 (2)

2.83 ± 0.25 (3)


Values: nmol/min/mg protein, mean ± SEM (n). Level of FBPase in cysQ mutants relative to parent strain is significantly different (P < 0.05; t-test). Level of FBPase in H37Rv parent strain reported in table 4 is significantly different (P < 0.05; t-test) to that reported in Table 3.

Movahedzadeh et al. BMC Microbiology 2010 10:50   doi:10.1186/1471-2180-10-50

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